At the end of 2022, an international multicentre, double-blind, randomised controlled efficacy and safety clinical trial involving a joint team of researchers from the Netherlands, Singapore, China and India was published in GeroScience:


Supplementation with NMN increases blood NAD+ levels and is effective in increasing physical performance, improving blood physiological age, and significantly improving health assessment scores.

NMN has shown positive effects on the human body in various aspects:

1. Increase blood NAD+ levels:

NMN is a precursor substance of NAD+, which can be converted into NAD+ after entering the human body. With age, the level of NAD+ in the human body will gradually decline, and supplementation of NMN can effectively improve the synthesis of NAD+, thus increasing the level of NAD+ in the blood.

This is important for maintaining normal cellular function and metabolism, as NAD+ plays a key role in many biological processes such as cellular energy metabolism, DNA repair, and regulation of gene expression.

In this study, compared with the control group, subjects taking different doses (300mg, 600mg, or 900mg) of NMN showed a significant increase in NAD+ levels in blood cells at the end of 60 days of treatment, with the 600mg dose group showing a more prominent increase.


2. Improvement of physical performance:

Enhancement of endurance performance: The 6-minute walking distance test revealed that subjects taking NMN showed significant improvement in physical performance. In general, 6-minute walking distance is an important indicator for assessing endurance and physical strength.

In the study, there was no significant increase in walking distance in the control and low-dose (300mg) groups, but the 600mg and 900mg dose groups showed a substantial increase in walking distance compared to baseline, suggesting that higher doses of NMN can enhance muscular endurance and improve physical performance.

Improvement of muscle function: NMN may affect energy metabolism and mitochondrial function in muscle cells by increasing NAD+ levels, which in turn affects muscle cell energy metabolism and mitochondrial function.


3. Improving blood physiological age:

The study used a tool called aging.ai 3.0 to assess physiological age, where participants simply entered data on height, weight, and 19 metrics such as cholesterol and haemoglobin to arrive at a predicted physiological age.

After 60 days, the physiological age of the control group increased, while in the NMN group, although the change in physiological age was not statistically significant in the 300mg and 900mg groups, the physiological age of the 600mg group decreased by about 1 year compared to the control group, suggesting that NMN may have a tendency to reverse physiological age to some extent.


4. Enhanced health assessment scores:

The SF-36 questionnaire was used in the study to measure subjects' well-being and overall feelings of health. The results showed that participants taking NMN had improved self-assessed health assessment scores after 30 and 60 days, indicating that they felt better about their health and well-being.

The increase in NAD+ levels in the blood and the improvement in physical fitness objectively reflected the subjects' improved physical functioning, which, when combined, resulted in improved scores on the health assessment questionnaire, reflecting the positive impact of NMN on overall health.